Real-time polymerase chain reaction techniques for SARS-CoV-2 nucleic acids were used to investigate the possible presence of the virus in vaginal discharge and amniotic fluid. It was performed by means of an amniocentesis in four Caucasian pregnant women during their second trimester of pregnancy who had an active infection confirmed by SARS-CoV-2, with associated mild symptoms. The time interval from the first positive SARS-CoV-2 RT-PCR result in nasopharyngeal specimens and the reported result in cervicovaginal and amniotic fluid specimens was 5, 7, 6, and 12 days, respectively. This study concludes that there is no evidence to suggest a possible passage of SARS-CoV-2 from the infected mother to the amniotic fluid. However, since the technique used does not turn out to be the one that demonstrates the best evidence, a negative result in the tests does not rule out the presence of infection and vertical transmission, for a more reliable result to demonstrate the intrauterine transmission of SARS-CoV-2 is required an unviable technique with which the presence and replication in fetal lung tissue is demonstrated, so it is necessary to expand the investigation of COVID-19 cases diagnosed during pregnancy to establish the real influence that SARS-CoV-2 has in pregnant women and their offspring, as well as those factors that intervene in the disease