Wang et al. compared the sensitivity of five RT-PCR detection kits for the SARS-CoV-2 virus and proposed strategies to reduce the risk of false negative results, especially for mixed sample detection. The authors reported that each kit had significantly different limits of detection, depending on the target gene of each kit. For example, the sensitivity for detection of the open reading frame 1ab gene (ORF1ab) was lowest compared to the nucleocapsid (N) and envelope (E) genes. It was suggested detection kits with lower sensitivity should be used in conjunction with a validation kit with higher sensitivity to reduce the risk of false negatives. For specimens with inconclusive results, such as amplification in a suspicious interval region,continuous amplification can be used to increase the detection rate of low viral load specimens and reduce the false negative rate of SARS-CoV-2 detection.