Aircrafts can increase an individual’s risk for exposure to SARS-CoV-2. Using a bacteriophage MS2 virus, the study assessed the exposure of SARS-CoV-2 for an aircraft with a single aisle (with two sections) and for an aircraft with a twin-aisle (with 3 sections). Findings showed that with physical distancing through keeping a middle seat unused, exposure was reduced by 23% in the single aisle scenario (one passenger sitting in the same row as another passenger but 2 seats away) compared to a full aircraft. There was also a 57% reduction in exposure in a twin-aisle scenario (different patterns of seating but always keeping the middle seat empty) compared to a full aircraft. When looking at relative exposure for one infectious passenger, exposure was reduced by 35%-36.4%. For two and three infectious passengers, relative exposure was reduced by 35.1%-38.2% and 35.9%-39.4%, respectively. The results of this study indicate that risk of exposure can be reduced by implementing physical distancing in aircrafts.